Fixable Viability eFluor^® Dyes have the significant advantage over conventional viability dyes of irreversibly labeling dead cells prior to the fixation and permeabilization steps that are required for staining intracellular targets.

These are amine-reactive fluorescent reagents, excited by the violet or red laser, that bind free amine groups on proteins. They are not membrane permeable and therefore only minimally label live cells. However, dead cells with compromised membranes allow access of the dye to the interior of the cell resulting in a very bright fluorescence of the dead cell population.

Unlike 7-AAD and Propodium Iodide, Fixable Viability eFluor^® Dyes are retained by dead cells throughout fixation, permeabilization and cryopreservation.

Excluding dead cells from analysis is a recommended procedure for all intracellular staining protocols including:

• detection of nuclear factors,
• transcription factors,
• cytosolic signaling molecules,
• secreted growth factors and cytokines.

 
Related products:

• Fixable Viability Dye eFluor^® 450
• Fixable Viability Dye eFluor^® 660
• Fixable Viability Dye eFluor^® 780