Use Cell Proliferation Dye eFluor^® 670 for tracking cell proliferation as an alternative to CFSE. This dye is ideally suited for situations where CFSE cannot be used due to the presence of FITC or GFP.

As with CFSE, Cell Proliferation Dye eFluor^® 670 accurately indicates cell division by partitioning equally into daughter cells during successive cell divisions, and can be used for both in vitro and in vivo tracking of cell proliferation.

Cell Proliferation Dye eFluor^® 670 is excited by the red laser and emits at 670 nm.


Cell Proliferation Dye eFluor^® 670 is a red fluorescent dye that can be used to monitor individual cell divisions.

This fluorescent dye binds to any cellular protein containing primary amines, and as cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye. Up to 6 generations may be visualized.

Cell Proliferation Dye eFluor^® 670 can also be used for long term tracking of labeled cells. Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation.

Cells labeled with Cell Proliferation Dye eFluor^® 670 may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers, such as the Foxp3 Staining Buffer Set (cat. 00-5523) or the IC Fixation Buffer (cat. 00-8222) and Permeabilization Buffer (10X) (cat. 00-8333).

Cell Proliferation Dye eFluor^® 670 has a peak excitation of 647 nm and can be excited by the red (633 nm) laser line. It has a peak emission of 670 nm and can be detected with a 660/20 band pass filter (equivanet to APC, Alexa Fluor® 647, or eFluor® 660), making it compatible with applications that utilize GFP.